Fereshteh Javadian; Zahra Sepehri; Mansour Amraee; Zohreh Kiani; Maryam Shahraki Mojahed; Zahra Shahi; Sepideh Pourghasemi Fetideh
Volume 22, Issue 4 , September and October 2015, , Pages 565-570
Abstract
Background and Purpose: The present study was carried out to determine the potential antibacterial agent of ethanolic extract of Hibiscus Sabdariffa against Klebsiella Pneumonia isolation of urinary tract infections. Methods and Materials:The leaf H. Sabdariffa was properly dried and pulverized ...
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Background and Purpose: The present study was carried out to determine the potential antibacterial agent of ethanolic extract of Hibiscus Sabdariffa against Klebsiella Pneumonia isolation of urinary tract infections. Methods and Materials:The leaf H. Sabdariffa was properly dried and pulverized into a coarse powder as described by Hanafy. Twenty grams of grinded powders from each plant were soaked in 60 ml of ethanol (95 %v/v) plus water, separately for one day with occasionally shaking. After one day of dissolving, materials were filtered through a Whatman no. 1 filter paper .Then the filtrates were evaporated using rotary evaporator. At last, 0.97 g of dried extract was obtained and then stored at40C in an air tight screw-cap tube. Isolation of bacteria : All 12 K. Pneumoniae were isolated from urine culture of hospitalized patients (Amir Al-Momenin Hospital, Zabol, south-eastern Iran), whom suffered from urinary tract infections during the years 2010- 2011. Isolated bacteria were identified by Gram's stain and standard biochemical tests. Sensitive strains to several antibiotics were evaluated by the disk diffusion method with standard Kirby - Bauer. Results: Results showed Klebsiella Pneumoniae isolates resistant to the antibiotics Erythromycin (75%) , Cefixime (3/58%) and Ceftazidime (3/33%). The results showed that the ethanol extract of Hibiscus tea with 10 ml / mg and l5 mg/m density have maximum inhibitory against Klebsiella Pneumonia samples and only one strain of K. Pneumoniae is inhibited in concentration 1/25. Discussion: It seems that H.Sabdariffa extracts could inhibit the growth of all of the mentioned bacteria. We noticed that the bactericidal effect of H.Sabdariffa extract was less than its bacteriostatic effects.